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AnOxP Prediction Pools
First, we use different enzymes (or combination enzymes) to simulate the hydrolysis of 21249 proteins from 60 species to obtain hydrolyzed fragments, and remove illegal sequences among them. Then, search the DFBP database to determine whether the obtained fragments are antioxidative peptides (AnOxPs), and screen out unreported sequences. Finally, use AnOxPP to predict these sequences, screen peptides with a certain probability range, and add them to the data pool.
Fragment statistics obtained by different hydrolysis methods
Enzyme(s) Probability Unique sequences Prediction Pools Download
Combine and filter the fragments obtained from the following nine hydrolysis methods
Reported by DFBP 395 reported_AnOxPs_all.txt download
No reported 2321342 unreported_fragments_all.txt download
< 0.50 548656 probability_all < 0.50.txt download
≥ 0.50 1772686 probability_all ≥ 0.50.txt download
≥ 0.90 1615584 probability_all ≥ 0.90.txt download
≥ 0.95 1542585 probability_all ≥ 0.95.txt download
Hydrolysis 1: Chymotrypsin +
Trypsin +
Pepsin (pH=1.3)
Reported by DFBP 133 reported_AnOxPs_ChyTryPep.txt download
No reported 313149 unreported_fragments_ChyTryPep.txt download
< 0.50 32034 probability_ChyTryPep < 0.50.txt download
≥ 0.50 281115 probability_ChyTryPep ≥ 0.50.txt download
≥ 0.90 265412 probability_ChyTryPep ≥ 0.90.txt download
≥ 0.95 256664 probability_ChyTryPep ≥ 0.95.txt download
Hydrolysis 2: Thermolysin +
Papain
Reported by DFBP 107 reported_AnOxPs_ThePap.txt download
No reported 154566 unreported_fragments_ThePap.txt download
< 0.50 53069 probability_ThePap < 0.50.txt download
≥ 0.50 101497 probability_ThePap ≥ 0.50.txt download
≥ 0.90 83355 probability_ThePap ≥ 0.90.txt download
≥ 0.95 74884 probability_ThePap ≥ 0.95.txt download
Hydrolysis 3: Pepsin +
Pancreatic
Reported by DFBP 95 reported_AnOxPs_PepPan.txt download
No reported 138379 unreported_fragments_PepPan.txt download
< 0.50 76506 probability_PepPan < 0.50.txt download
≥ 0.50 61873 probability_PepPan ≥ 0.50.txt download
≥ 0.90 48460 probability_PepPan ≥ 0.90.txt download
≥ 0.95 43292 probability_PepPan ≥ 0.95.txt download
Hydrolysis 4: Proteinase K
Reported by DFBP 67 reported_AnOxPs_ProteinaseK.txt download
No reported 188918 unreported_fragments_ProteinaseK.txt download
< 0.50 76270 probability_ProteinaseK < 0.50.txt download
≥ 0.50 112648 probability_ProteinaseK ≥ 0.50.txt download
≥ 0.90 95463 probability_ProteinaseK ≥ 0.90.txt download
≥ 0.95 88849 probability_ProteinaseK ≥ 0.95.txt download
Hydrolysis 5: Thermolysin
Reported by DFBP 113 reported_AnOxPs_Thermolysin.txt download
No reported 431224 unreported_fragments_Thermolysin.txt download
< 0.50 92560 probability_Thermolysin < 0.50.txt download
≥ 0.50 338664 probability_Thermolysin ≥ 0.50.txt download
≥ 0.90 301105 probability_Thermolysin ≥ 0.90.txt download
≥ 0.95 281660 probability_Thermolysin ≥ 0.95.txt download
Hydrolysis 6: Papain
Reported by DFBP 158 reported_AnOxPs_Papain.txt download
No reported 336030 unreported_fragments_Papain.txt download
< 0.50 113717 probability_Papain < 0.50.txt download
≥ 0.50 222313 probability_Papain ≥ 0.50.txt download
≥ 0.90 195640 probability_Papain ≥ 0.90.txt download
≥ 0.95 183137 probability_Papain ≥ 0.95.txt download
Hydrolysis 7: Pepsin (pH=1.3)
Reported by DFBP 268 reported_AnOxPs_Pepsin.txt download
No reported 437396 unreported_fragments_Pepsin.txt download
< 0.50 112805 probability_Pepsin < 0.50.txt download
≥ 0.50 324591 probability_Pepsin ≥ 0.50.txt download
≥ 0.90 293809 probability_Pepsin ≥ 0.90.txt download
≥ 0.95 279517 probability_Pepsin ≥ 0.95.txt download
Hydrolysis 8: Chymotrypsin
Reported by DFBP 129 reported_AnOxPs_Chymotrypsin.txt download
No reported 458355 unreported_fragments_Chymotrypsin.txt download
< 0.50 73080 probability_Chymotrypsin < 0.50.txt download
≥ 0.50 385275 probability_Chymotrypsin ≥ 0.50.txt download
≥ 0.90 354989 probability_Chymotrypsin ≥ 0.90.txt download
≥ 0.95 340481 probability_Chymotrypsin ≥ 0.95.txt download
Hydrolysis 9: Trypsin
Reported by DFBP 138 reported_AnOxPs_Trypsin.txt download
No reported 386104 unreported_fragments_Trypsin.txt download
< 0.50 53050 probability_Trypsin < 0.50.txt download
≥ 0.50 333054 probability_Trypsin ≥ 0.50.txt download
≥ 0.90 321800 probability_Trypsin ≥ 0.90.txt download
≥ 0.95 317554 probability_Trypsin ≥ 0.95.txt download
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