Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1325(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1325
Peptide sequence	AKYSY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ala-Lys-Tyr-Ser-Tyr

Single-letter amino acid

AKYSY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
631.5 Da	630.69 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.52 ^c

IC₅₀

2.42 uM

pIC₅₀

-0.384

GRAVY

-1.1000^c

Hydrophilic residue ratio

20%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Marine, Plant
Organism/Source	Laminaria japonica
Precursor protein	Laminaria japonica hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1035 ---- Plant proteins ---- Probable L-ascorbate peroxidase 8, chloroplastic Source.2: DFBPPR1480 ---- Plant proteins ---- CASP-like protein BLE3 Source.3: DFBPPR5989 ---- Plant proteins ---- CASP-like protein 1C2 Source.4: DFBPPR14346 ---- Marine protein ---- R-phycoerythrin alpha chain Source.5: DFBPPR7860 ---- Plant protein ---- CASP-like protein 1C1
Link-research Inductive analysis	Link 1: DFBPACEI0786----Red algae----Red algae protein Link 2: DFBPACEI2220----Laminaria japonica----Laminaria japonica proteins (LJPs)

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited strong Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 2.42 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Laminaria japonica protein was hydrolyzed with combined enzymes (alcalase, trypsin and papain).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

(1) Laminaria japonica proteins would be a good source of hypotensive peptides.
(2) A simple and efficient RP-HPLC method was developed for simultaneous analysis of eight small anti-hypertensive peptides with tyrosine at the C-terminal.

Database cross-references

DFBP

[D1]	DFBPANHY0661
[D2]	DFBPMUFU0173

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Chen, J.-C., Wang, J., Zheng, B.-D., Pang, J., Chen, L.-J., Lin, H.-t., et al. Simultaneous Determination of 8 Small Antihypertensive Peptides with Tyrosine at the C-Terminal inLaminaria japonicaHydrolysates by RP-HPLC Method. Journal of Food Processing and Preservation. 2016, 40, 492-501. DOI: 10.1111/jfpp.12628
Other literature(s)	N.D
PubDate	2016