Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPPEPI0056(PEP-inhibitory peptide)

DFBP ID	DFBPPEPI0056
Peptide sequence	IYPF
Type	Native peptide
Peptide/Function name	PEP-inhibitory peptide

Function-activity relationship

Main bioactivity	PEP-inhibitory activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antioxidative activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Ile-Tyr-Pro-Phe

Single-letter amino acid

IYPF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	538.63 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

1.1000^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human milk protein
Precursor protein	β-Casein
Residue position	f(50-53)
Precursor protein(s) search	Source.1: DFBPPR1008 ---- Plant proteins ---- Cellulose synthase A catalytic subunit 9 [UDP-forming] Source.2: DFBPPR1414 ---- Plant proteins ---- Cellulose synthase-like protein D1 Source.3: DFBPPR2091 ---- Plant proteins ---- Cellulose synthase-like protein D3 Source.4: DFBPPR2105 ---- Plant proteins ---- Cellulose synthase-like protein D2 Source.5: DFBPPR2121 ---- Plant proteins ---- Cellulose synthase-like protein D5 Source.6: DFBPPR2171 ---- Plant proteins ---- Probable mixed-linked glucan synthase 8 Source.7: DFBPPR7398 ---- Plant proteins ---- 1-acyl-sn-glycerol-3-phosphate acyltransferase BAT2, chloroplastic Source.8: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.9: DFBPPR15999 ---- Animal proteins ---- Prostaglandin E synthase Source.10: DFBPPR17020 ---- Animal proteins ---- Prostaglandin E synthase Source.11: DFBPPR19363 ---- Animal proteins ---- Ethanolaminephosphotransferase 1 Source.12: DFBPPR19880 ---- Animal proteins ---- TATA box-binding protein-like 1 Source.13: DFBPPR21141 ---- Animal proteins ---- Biotinidase Source.14: DFBPPR10411 ---- Animal proteins ---- DNA-dependent protein kinase catalytic subunit Source.15: DFBPPR11504 ---- Animal proteins ---- TATA box-binding protein-like 1 Source.16: DFBPPR13177 ---- Animal proteins ---- Prostaglandin E synthase Source.17: DFBPPR13866 ---- Animal proteins ---- Type-2 angiotensin II receptor Source.18: DFBPPR15768 ---- Microorganism protein ---- Pheromone-regulated membrane protein 10 Source.19: DFBPPR8104 ---- Plant protein ---- DNA-directed RNA polymerase subunit beta
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

PEP-inhibitory activity	Inhibitor of Prolyl endopeptidase (EC:3.4.21.26). The peptide possibly act as anti-amnestic agents. The peptide was assayed in vitro for their capacity to inhibit the Flavobacterium meningosepticum PEP activity, the IC₅₀ value is 36 uM and it's moderately active.
Specific target protein(s)	Specific Target Protein(s): Prolyl endopeptidase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Synthetic peptide
Enzyme(s)/starter culture	No enzyme

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI1675
[D2]	DFBPANOX0705
[D3]	DFBPMUFU0479

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Asano M, Nio N, Ariyoshi Y. Inhibition of prolyl endopeptidase by synthetic peptide fragments of human beta-casein. Agric Biol Chem. 1991 Mar;55(3):825-8. PMID: 1368631
Other literature(s)	N.D
PubDate	1991